Pharmcia（GE）葡聚糖凝膠G-75
Pharmcia（GE)葡聚糖凝膠G-75
Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Pharmcia（GE)葡聚糖凝膠G-75
| Matrix | Cross-linked dextran |
| Particle Size Wet | 90 µm-280 µm |
| Partical Size Dry | 40 µm-120 µm |
| pH Stability Working Range | 2-10 |
| Flow Rate | < Darcy´s Law1) |
| Pressure/Flow Specification | min 90 cm/h, bed height 10 cm, column 5 cm i.d. |
| Fractionation [Mr] Globular Proteins | 3 x 103 – 8 x 104 |
| Fractionation [Mr] Dextrans | 1 x 103-5 x 104 |
| Exclusion Limit | > 7 x 104 |
| Storage Conditions | 4 to 30°C |
| Autoclavable | In wet form (pH 7.0) at 120°C for 30 min |
| 1)The beads behave as rigid spheres obeying Darcy's Law. | |
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