Pharmcia（GE)葡聚糖凝膠G-15Sephadex G-15

Sephadex G-15, 100 g

Sephadex G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1 500 molecular weight.

Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 1 500 molecular weight
Efficient removal of contaminants such as salts, dyes, and radioactive labels

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

Pharmcia（GE)葡聚糖凝膠G-15Sephadex G-15

Sephadex G-15, 100 g

Matrix	Cross-linked dextran
Particle Size Wet	60 µm-180 µm
Partical Size Dry	40 µm-120 µm
pH Stability Working Range	2-13
Flow Rate	< Darcy´s Law¹⁾
Pressure/Flow Specification	20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d.
Fractionation [Mr] Globular Proteins	1.5 x 10³
Fractionation [Mr] Dextrans	>< 1.5 x 10³
Storage Conditions	4 to 30°C
Autoclavable	In wet form (pH 7.0) at 120°C for 30 min
¹⁾The beads behave as rigid spheres obeying Darcy's Law.

Sephadex G-15, 100 g

Handbook
Size Exclusion Chromatography: Principles and Methods Summary This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close

Handbook

Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close

Magazine
Upstream Downstream issue 2 Close

Material Safety Data Sheet
Sephadex™ G-15, 100 g Close
Sephadex™ G-15, 100 g Close

Selection guide
Protein and Peptide Purification: Technique selection guide Summary This technique selection guide is designed to help you in selecting the technique most suited to your application. It covers ion exchange chromatography (IEX), reversed phase chromatography (RPC), hydrophobic interaction chromatography (HIC), gel filtration (GF), and affinity chromatography (AC). Close

Pharmcia（GE)葡聚糖凝膠G-15Sephadex G-15 詳細產品信息可和選購